Synergistic reduction of pollution and carbon mitigation in constructed wetlands-microbial fuel cell using sludge-derived biochar.

Integrating microbial fuel cells (MFC) into constructed wetland systems (CW) has been an efficient wastewater treatment to improve the pollutants removal and regenerate power energy. This study fabricated a sludge biochar material (SBM) to sequestrate the carbon of residual sludge. Thereafter used SBM and modified SBM as the substrate materials to construct three groups of CW-MFC for decreasing the greenhouse gas (GHG) emission. The water quality improvement in removal efficiency achieved (2.59 %, 3.10 %, 5.21 % for COD; 3.31 %, 3.60 %, 6.71 % for TN; 1.80 %, 7.38 %, 4.93 % for TP) by the application of MFC, SBM, and modified SBM in wastewater treatment, respectively. Additionally, the reduction in global warming potential (GWP) realized 17.2 %, 42.2 %, and 64.4 % resulting from these applications. The carbon flow and fate diagrams showed MFC shifted the gas phase­carbon flow from CH4 to CO2, and SBM promoted this shift trends. Microbial diversity indicated enrichment of electrochemically active bacteria (EAB), denitrifying bacteria, and phosphate accumulating organisms (PAOs) by SBM. Metabolic pathways analysis showed that introduction of MFC and SBM exhibited significant increases of key functional genes in metabolic pathway of anaerobic oxidation of methane (AOM). This study highlights the benefit of CW-MFC in and provides a new strategy for removing pollutants and abating GHG emissions in wastewater treatment.

Performance and mechanism of sacrificed iron anode coupled with constructed wetlands (E-Fe) for simultaneous nitrogen and phosphorus removal.

Three anodic biofilm electrode coupled CWs (BECWs) with graphite (E-C), aluminum (E-Al), and iron (E-Fe), respectively, and a control system (CK) were constructed to evaluate the removal performance of N and P in the secondary effluent of wastewater treatment plants (WWTPs) under different hydraulic retention time (HRT), electrified time (ET), and current density (CD). Microbial communities, and different P speciation, were analyzed to reveal the potential removal pathways and mechanism of N and P in BECWs. Results showed that the optimal average TN and TP removal rates of CK (34.10% and 55.66%), E-C (66.77% and 71.33%), E-Al (63.46% and 84.93%), and E-Fe (74.93% and 91.22%) were obtained under the optimum conditions (HRT 10 h, ET 4 h, CD 0.13 mA/cm2), which demonstrated that the biofilm electrode could significantly improve N and P removal. Microbial community analysis showed that E-Fe owned the highest abundance of chemotrophic Fe(II) (Dechloromonas) and hydrogen autotrophic denitrifying bacteria (Hydrogenophaga). N was mainly removed by hydrogen and iron autotrophic denitrification in E-Fe. Moreover, the highest TP removal rate of E-Fe was attributed to the iron ion formed on the anode, causing co-precipitation of Fe(II) or Fe(III) with PO43--P. The Fe released from the anode acted as carriers for electron transport and accelerated the efficiency of biological and chemical reactions to enhance the simultaneous removal of N and P. Thus, BECWs provide a new perspective for the treatment of the secondary effluent from WWTPs.

Revealing the microbial mechanism of Fe0 and MnO2 mediated microbial fuel cell-anaerobic digestion coupling system and its energy flow distribution.

Microbial fuel cell-anaerobic digestion (MFC-AD) is a new sludge treatment technology with multi-path energy recovery. In this study, Fe0 and MnO2 with gradient concentration were added to investigate its effects on the sludge reduction, electrochemical performance, extracellular polymeric substances (EPS) of sludge, microbial community, electron distribution and energy flow of the MFC-AD system. Results showed that the highest sludge reduction 59% (49%), was obtained at 10 g/L Fe0 (5 g/L MnO2) adding and its total energy recovery efficiency increased by 100% (71%) compare to the control. Different Fe0 and MnO2 concentrations lead to different microbial mechanisms: at 10 g/L Fe0 or 5 g/L MnO2, it prefers to promote extracellular electrons transfer, favoring the Geobacter, Shewanella and Acinetobacter enrichment, while at 5 g/L Fe0 or 0.5 g/L MnO2 it plays a more important role in substrate metabolism of anaerobic digestion, with Clostridium, Roseomonas lacus, and Methylocystis enriched. Correspondingly, the electron quantity distribution from biomass to recovered energy ends (Current, CH4 and VFAs), was influenced by Fe0 and MnO2 concentration, indicating the controllability of the energy flow.

The performance and mechanism of iron-modified aluminum sludge substrate tidal flow constructed wetlands for simultaneous nitrogen and phosphorus removal in the effluent of wastewater treatment plants.

Aiming at the poor N and P removal performance in the effluent of wastewater treatment plants by constructed wetlands (CWs), aluminum sludge (AS) from water supply plants was used to prepare iron-modified aluminum sludge (IAS), and tidal flow constructed wetlands (TFCWs) using IAS as substrates were constructed. By means of high-throughput sequencing, X-ray diffractometer (XRD), etc., the removal mechanism of N and P in the system and fate analysis of key elements were also interpreted. Results showed that an interlayer structure beneficial to adsorbing pollutants was formed in the IAS, due to the iron scraps entering into the molecular layers of AS. The removal rates of TP and TN by IAS-TFCWs reached 95 % and 47 %, respectively, when the flooding/resting time (F/R) and C/N were 6 h/2 h and 6. During the three-year operation of the IAS-TFCWs, the effluent concentrations of CODCr, NH4+-N, and TP could comply with Class IV Standard of "Environmental Quality Standards for Surface Water" (GB3838-2002). The mechanism analysis showed that the N removal was effectuated through Fe2+ as the electron donor of Fe(II)-driven the autotrophic denitrifying bacteria to reduce nitrate, while the P removal mainly depended on the adsorption reaction between FeOOH in IAS and phosphate. In conclusion, the stable Fe-N cycle in the IAS-TFCWs achieved simultaneous and efficient N and P removal.

Mechanism of Fe-C micro-electrolysis substrate to improve the performance of CW-MFC with different factors: Insights of microbes and metabolic function.

Constructed wetland-microbial fuel cell (CW-MFC) is a novel technology for wastewater treatment with electrical generation. This work proposed a Fe-C micro-electrolysis substrate (Fe-C) with biomass modified ceramsite to enhance pollutants removal and electricity generation. The key influencing factors were revealed, and the COD, NH4+-N, and TP removal efficiency was respectively increased by 10.2, 8.1 and 8.78% with 76% higher power output at optimal conditions (e.g. OLR 52.5 g/(m2.d), HRT 48 h, and aeration rate 800 mL/min). Fe-C based substrates improved the microenvironments in CW-MFC, including dissolved oxygen (DO) and oxidation-reduction potential (ORP) lowering and electron transfer facilitation. These contributed to the enrichment of critical microorganisms and metabolic activities. The abundance of functional bacteria (i.e. Geobacter, Thauera and Dechloromonas) were evidently increased. Additionally, the energy metabolism and other functional genes encoding cytochrome c (ccoN), nitrite reductase (nirD) and phosphate transporter (pstA) were all stimulated.